initiator trna การใช้

• IF2 binds to an initiator tRNA and controls the entry of that tRNA into the ribosome.
• Folates are  conjugated pterins that contain " N "-formylmethionine in initiator tRNAs.
• SUI1 is a translation start site, helped by eIF-2 and the initiator tRNA-Met.
• It has been suggested that after binding of the initiator tRNA to the P / I site, it is correctly positioned to the P site by binding of EF-P to the E site.
• The initiator tRNA occupies the P site in the ribosome, and the A site is ready to receive an aminoacyl-tRNA . During chain elongation, each additional amino acid is added to the nascent polypeptide chain in a three-step microcycle.
• This is important in purine biosynthesis, where 10-CHO-THF is a substrate for phosphoribosylaminoimidazolecarboxamide formyltransferase, as well as in the formylation of the methionyl initiator tRNA ( fMet-tRNA ), when 10-CHO-THF is a substrate for methionyl-tRNA formyltransferase.
• IF3 is not universally found in all bacterial species but in " E . coli " it is required for the 30S subunit to bind to the initiation site in mRNA . In addition, it has several other jobs including stabilization of free 30S subunits, facilitation of 30S subunits binding to mRNA and checking for accuracy against the first aminoacyl-tRNA . It also allows for rapid codon-anticodon pairing for the initiator tRNA to bind quickly to.